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微信小章| 产品名称： | Dengue virus type 1 |
|---|---|
| 商品货号： | TS213778 |
| Classification： | Flaviviridae, Flavivirus |
| Agent： | Dengue virus type 1 |
| Strain： | Hawaii |
| Applications： | Vector-borne disease research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of mouse-prepared product ATCC® VR-1254™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1254™ and ATCC® VR-1856™ have not been compared for sequence or infectivity in tissue culture and in vivo. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Vero cells (ATCC® CCL-81™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.02). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 7-15 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | R Selvarangan, Children’s Mercy Hospital |
| Source： | Pooled serum from 6 patients, Hawaii, 1944 |
| References： | Sabin AB. . Science 101: 640, 1945. Sabin AB. Viral and Rickettsial Infections of Man. Philadelphia: JB Lippincott Co; 1959. |