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微信小章| 产品名称： | Viral hemorrhagic septicemia virus |
|---|---|
| 商品货号： | TS213779 |
| Classification： | Rhabdoviridae, Novirhabdovirus, Piscine novirhabdovirus |
| Agent： | Viral hemorrhagic septicemia virus |
| Strain： | Makah |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of CHSE (ATCC® CRL-1681™) cell culture product ATCC® VR-1387™ to EPC cells (ATCC® CRL-2872™). ATCC® VR-1387™ and ATCC® VR-1872™ have not been compared for sequence or infectivity. |
| Effect on Host： | CPE, refractile rounding and degeneration |
| Recommended Host： | Epithelioma Papulosum Cyprini (EPC) cells (ATCC® CRL-2872™) |
| Growth Conditions： | Temperature: 15°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 15°C in a humidified atmosphere. End adsorption by adding virus growth medium. Incubation: 2-4 days at 15°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Winton |
| Source： | Isolated by R. Brunson from ovarian fluid of normal adult coho salmon returning in 1988 to the Makah National Fishery, WA. |
| References： | Bernard J, et al. Nucleocapsid gene sequence of a North American isolate of viral haemorrhagic septicaemia virus, a fish rhabdovirus. J. Gen. Virol. 73: 1011-1014, 1992. PubMed: 1634868 Winton JR. . Proc. 2nd Int. Symp. Viruses Lower Verts : 43-50, 1991. |