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| 产品名称： | Human rhinovirus 50 |
|---|---|
| 商品货号： | TS213780 |
| Classification： | Picornaviridae, Enterovirus, Human rhinovirus A |
| Deposited As： | Rhinovirus 50 |
| Agent： | Human rhinovirus 50 |
| Strain： | A2#58 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | ATCC® VR-1876™ was derived from ATCC® VR-517™ following treatment with diethyl ether to remove mycoplasma contamination. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | HeLa cells (ATCC® CCL-2™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1.5-2.0 hours at 33°C in a humidified 5% CO2 atmosphere while rocking. End adsorption by adding virus growth medium. Incubation: 6-7 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | VV Hamparian, RM Conant |
| Chain of Custody： | ATCC |
| Source： | Human throat washings |