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微信小章| 产品名称： | Human rhinovirus 48 |
|---|---|
| 商品货号： | TS213782 |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus B |
| Deposited As： | Rhinovirus 48 |
| Agent： | Human rhinovirus 48 |
| Strain： | 1505 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | ATCC® VR-1875™ was derived from ATCC® VR-515™ following treatment with diethyl ether to remove mycoplasma contamination. Recognized Reference Strain: Collaborative Report on Rhinoviruses, Nature (Lond.) 213: 761, 1967. |
| Effect on Host： | CPE, refractile rounding and sloughing with cell degeneration |
| Recommended Host： | WI-38 cells (ATCC® CCL-75™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 1.0). Adsorb 1.5-2.0 hours at 33°C in a humidified 5% CO2 atmosphere on a rocking platform. End adsorption by adding virus growth medium. Incubation: 3-5 days at 33°C in a humidified 5% CO2 atmosphere on a rocking platform, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | VV Hamparian, RM Conant, Ohio State University |
| References： | Kapikian A, et al. Characterization of two newly recognized rhinovirus serotypes of human origin. Proc. Soc. Exp. Biol. Med. 122: 1155-1162, 1966. PubMed: 4288216 Kapikian AZ et al. Rhinoviruses: a numbering system. Nature 213: 761-762, 1967. PubMed: 4291698 |