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微信小章| 产品名称： | Human echovirus 2 |
|---|---|
| 商品货号： | TS213784 |
| Classification： | Picornaviridae, Enterovirus, Human enterovirus B |
| Deposited As： | Echovirus 2 |
| Agent： | Human echovirus 2 |
| Strain： | Cornelis |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of primary cell culture product ATCC® VR-1039™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1867™ and ATCC® VR-1039™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Vero cells (ATCC® CCL-81™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 16-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 4-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | NIH/NIAID |
| Source： | Feces from a patient with aseptic meningitis, Connecticut, 1951. |
| References： | Melnick J. Application of tissue culture methods to epidemiological studies of poliomyelitis. Am. J. Public Health 44: 571-580, 1954. PubMed: 13148397 |