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微信小章| 产品名称： | Bovine popular stomatitis virus |
|---|---|
| 商品货号： | TS213785 |
| Classification： | Poxviridae, Chordopoxvirinae, Parapoxvirus |
| Agent： | Bovine popular stomatitis virus |
| Strain： | Illinois 721 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of primary cell culture product ATCC® VR-801™ to BT cells (ATCC® CRL-1390™). ATCC® VR-1855™ and ATCC® VR-801™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. Ensure that FBS is free of BVDV prior to use with BT cells. |
| Effect on Host： | CPE, refractile rounding and cell degeneration |
| Recommended Host： | BT cells (ATCC® CRL-1390™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2-3 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | RA Crandell, University of Illinois |
| Source： | Esophagus from a bovine calf with debilitating disease, ulcers on esophagus |
| References： | Crandell RA, Gosser HS. Ulcerative esophagitis associated with poxvirus infection in a calf. J. Am. Vet. Med. Assoc. 165: 282-283, 1974. PubMed: 4367640 Crandell RA, Conroy JD. . Ann. Proc. Vet. Lab. Diag. : 223-234, 1974. |