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微信小章| 产品名称： | Bovine respiratory syncytial virus |
|---|---|
| 商品货号： | TS213786 |
| Classification： | Pneumoviridae, Orthopneumovirus |
| Agent： | Bovine respiratory syncytial virus |
| Strain： | A 51908 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | ATCC® VR-1861™ was derived from ATCC® VR-794™ following treatment with Mycoplasma Removal Agent (MRA) to remove Mycoplasma contamination. |
| Effect on Host： | CPE, Syncytia with cell sloughing/detachment |
| Recommended Host： | BT cells (ATCC® CRL-1390™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer. |
| Name of Depositor： | SB Mohanty |
| Source： | Trachea of calf with respiratory disease |
| References： | Mohanty SB, et al. Experimentally induced respiratory syncytial viral infection in calves. Am. J. Vet. Res. 36: 417-419, 1975. PubMed: 164807 |