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微信小章| 产品名称： | Mayaro virus |
|---|---|
| 商品货号： | TS213787 |
| Classification： | Togaviridae, Alphavirus |
| Agent： | Mayaro virus |
| Strain： | TRVL 15537 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of suckling mouse product ATCC® VR-1277™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1277™ and ATCC® VR-1863™ have not been compared for sequence or infectivity in suckling mice, primary cell culture, or tissue culture. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Vero cells (ATCC® CCL-81™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 1-2 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | NIH/NIAID |
| References： |
Mavian C, et al. Emergence of recombinant Mayaro virus strain from the Amazon basin. Sci Rep 7: 8718, 2017. Long KC, et al. Experimental transmission of Mayaro virus byxa0Aedes aegypti. Am J Trop Med Hyg 85(4): 750-757, 2011. PubMed: 21976583 Raimunda SS, et al. Mayaro Fever Virus, Brazilian Amazon. Emerg Infect Dis 15(11): 1830-1832, 2009. Lavergne A, et al. Mayaro virus: complete nucleotide sequence and phylogenetic relationships with other alphaviruses. Virus Res 117(2): 283-290, 2006. PubMed: 16343676 |