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微信小章| 产品名称： | Usutu virus |
|---|---|
| 商品货号： | TS213788 |
| Classification： | Flaviviridae, Flavivirus |
| Agent： | Usutu virus |
| Strain： | ENT MP 1626 |
| Applications： | Vector-borne disease research Emerging infectious disease research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Arbovirus |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Vero E6 cells (ATCC® CRL-1586™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01 to 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 7-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | B Russell, CDC |
| Source： | Isolated from a mosquito in the Zika forest, Entebbe area, Uganda, 1962 |
| References： | Williams MC, et al. The Isolation of West Nile Virus from Man and of Usutu Virus from the Bird-Biting Mosquito Mansonia aurites (Theobald) in the Entebbe Area of Uganda. Ann Trop Med Parasitol 58: 367-374, 1964. PubMed: 14212897 Ashraf U, et al. Usutu Virus: An Emerging Flavivirus in Europe. Viruses 7: 219-238, 2015. PubMed: 25606971 Tetro JA. Is Usutu Virus Ready for Prime Time? Microbes Infect 19: 380-381, 2017. PubMed: 28619684 Pecorari M, et al. First Human Case of Usutu Virus Neuroinvasive Infection, Italy, August-September 2009. Euro Surveill 14(50): 19446, 2009. PubMed: 20070936 Santini M, et al. First Cases of Human Usutu Virus Neuroinvasive Infection in Croatia, August-September 2013: Clinical and Laboratory Features. J Neurovirol 21: 92-97, 2015. PubMed: 25361698 Gaibani P, et al. Comparative Genomic and Phylogenetic Analysis of the Usutu Virus Isolate from a Human Patient Presenting with Neurological Symptoms. PLoS One 8: e64761, 2013. PubMed: 23741387 |