微信小陈
微信小章
产品简介
购买须知
| 产品名称： | Human echovirus 9 |
|---|---|
| 商品货号： | TS213789 |
| Classification： | Picornaviridae, Enterovirus, Enterovirus B |
| Deposited As： | Echovirus 9 |
| Agent： | Human echovirus 9 |
| Strain： | Vispo |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of primary cell culture product ATCC® VR-1051™ to LLC-MK2 Derivative cells (ATCC® CCL-7.1™). ATCC® VR-1888™ and ATCC® VR-1051™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. The original source material has been reported to contain Simian virus 40. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | LLC-MK2 Derivative cells (ATCC® CCL-7.1™) |
| Growth Conditions： | Temperature: 36°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 36°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 3-4 days at 36°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer. |
| Name of Depositor： | NIH/NIAID |