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微信小章| 产品名称： | Macaca mulatta polyomavirus 1 |
|---|---|
| 商品货号： | TS213800 |
| Classification： | Polyomaviridae, Betapolyomavirus |
| Deposited As： | Macaca mulatta polyomavirus 1 |
| Agent： | Macaca mulatta polyomavirus 1 |
| Strain： | K661 |
| Common Name： | Simian virus 40, SV40 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Protoarchetype regulatory region; naturally severely attenuated from microRNA expression. SV40 regulatory region, nucleotides 5192-370; E = enhancer region within regulatory region; 1E = single 72-bp enhancer (archetypal); 2E = duplication/rearrangement in enhancer region (complex), nucleotides 107-294; G/C-rich region within regulatory region, nucleotides 40-103. Nucleotide numbers from SV40 reference strain 776-2E. |
| Effect on Host： | CPE, vacuolization, rounding, and sloughing |
| Recommended Host： | CV-1 cells (ATCC® CCL-70™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 9-16 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Butel, Baylor College of Medicine |
| Source： | Brain tissue from an SIV-infected rhesus monkey, Houston, Texas, USA, 1997 |
| References： | Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685 Forsman ZH, et al.xa0 Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation.xa0 J Virol 78(17): 9306-9316, 2004.xa0 PubMed: 15308725 Chen CJ, et al.xa0 Naturally arising strains of polyomaviruses with severely attenuated microRNA expression. J Virol 88(21):12683-12693, 2014. PubMed: 25142594 |