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微信小章| 产品名称： | Mumps rubulavirus |
|---|---|
| 商品货号： | TS213802 |
| Classification： | Paramyxoviridae, Rubulavirus |
| Agent： | Mumps rubulavirus |
| Strain： | MuV/Iowa.US/2006 (Genotype G) |
| Common Name： | Mumps virus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Mumps virus strain MuV-IA, complete genome |
| Images： |  |
| Effect on Host： | CPE, cell fusion and sloughing |
| Recommended Host： | Vero E6 cells (ATCC® CRL-1586™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.001-0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 3-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | P Rota, CDC |
| Source： | Isolated by oral swab from a human in Iowa, USA, 2006 |
| References： | Boddicker JD, et al. Real-time reverse transcription-PCR assay for detection of mumps virus RNA in clinical specimens.xa0 J Clin Microbiol. 45(9): 2902-2908, 2007.xa0 PubMed: 17652480 Xu P, et al. Rescue of wild-type mumps virus from a strain associated with recent outbreaks helps to define the role of the SH ORF in the pathogenesis of mumps virus.xa0 Virology 417(1): 126-136, 2011.xa0 PubMed: 21676427 |
| Cross References： | Nucleotide (GenBank) : JN012242.1 Mumps virus strain MuV-IA, complete genome Nucleotide (GenBank) : DQ661745.1 Mumps virus strain MUM/Iowa.US/2006 small hydrophobic protein mRNA, complete cds |