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微信小章| 产品名称： | Macaca mulatta polyomavirus 1 |
|---|---|
| 商品货号： | TS213804 |
| Classification： | Polyomaviridae, Betapolyomavirus |
| Deposited As： | Macaca mulatta polyomavirus 1 |
| Agent： | Macaca mulatta polyomavirus 1 |
| Strain： | Baylor SVB2E (WT), SVB |
| Common Name： | Simian virus 40, SV40 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | C-terminal variable domain of T-antigen differs from that of SV40-776 |
| Effect on Host： | CPE, vacuolization, rounding, and sloughing |
| Recommended Host： | CV-1 cells (ATCC® CCL-70™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 9-16 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Butel, Baylor College of Medicine |
| Source： | Houston, Texas, USA, 1961 |
| References： | Melnick JL, Stinebaugh S.xa0 Excretion of vacuolating SV-40 virus (papova virus group) after ingestion as a contaminant of oral poliovaccine.xa0 Proc Soc Exp Biol Med 109:965-968, 1962.xa0 PubMed: 14472428 Stinebaugh S, Melnick JL.xa0 Plaque formation by vacuolating virus, SV40. Virology 16: 348-349, 1962. PubMed: 13917252 Lanford RE, Butel JS.xa0 Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen. Cell 37(3):801-813, 1984. PubMed: 6086146 Stewart AR, et al.xa0 Identification of a variable region at the carboxy terminus of SV40 large T-antigen. Virology 221(2):355-361, 1996. PubMed: 8661447 Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047 Forsman ZH, et al.xa0 Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation.xa0 J Virol 78(17): 9306-9316, 2004.xa0 PubMed: 15308725 Sroller V, et al.xa0 Influence of the viral regulatory region on tumor induction by simian virus 40 in hamsters. J Virol 82(2):871-879, 2008. PubMed: 17977966 Patel NC, et al.xa0 Viral regulatory region effects on vertical transmission of polyomavirus SV40 in hamsters. Virology 386(1):94-101, 2009. PubMed: 19181358 |