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微信小章| 产品名称： | Influenza B virus (Victoria Lineage) |
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| 商品货号： | TS213808 |
| Classification： | Orthomyxoviridae, Influenzavirus B |
| Deposited As： | Influenza B virus |
| Agent： | Influenza B virus (Victoria Lineage) |
| Strain： | B/Florida/78/2015 (propagated in cell culture) |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 0.5 mL per vial |
| Storage Conditions： | -70°C or colder |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | MDCK cells (ATCC® CCL-34™) |
| Growth Conditions： | Temperature: 35°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1 to 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2-3 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 75% of the monolayer. |
| Name of Depositor： | Florida Department of Health |
| Source： | Isolated from a human in Florida, USA on November 09, 2015 |
| References： | Influenza Research Database – Details for B/Florida/78/2015 |