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微信小章| 产品名称： | Human astrovirus |
|---|---|
| 商品货号： | TS213812 |
| Classification： | Astroviridae, Mamastrovirus, Mamastrovirus 1 |
| Agent： | Human astrovirus |
| Strain： | Type 1 |
| Applications： | Virucide testing In vitro infectivity assay Antiviral Studies Spiking studies ELISpot Assay Virus neutralization assay Virus ultrastructural studies Immunological-based assays PCR-based assay validation Research and development Assay development Vaccine development |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Freeze-thaw twice at harvest. |
| Effect on Host： | No visible CPE – confirm infection by IFA. Sloughing and detachment may result from trypsin supplemented growth medium. |
| Recommended Host： | CaCo-2 cells (ATCC® HTB-37™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 90-100% confluent. Pre-treat inoculum in EMEM (ATCC® 30-2003™) + 5 µg/mL 1X-S trypsin (Sigma-Aldrich® T0303) for 30 minutes at 37°C. Remove medium from host cells and rinse twice with 1X DPBS. Inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1.0). Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by removing inoculum and adding virus growth medium. Incubation: 1-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Vinje, CDC, Division of Viral Diseases |
| Source： | Human stool sample, Oxford, UK |
| References： | Lee TW, Kurtz JB. Prevalence of human astrovirus serotypes in the Oxford region 1976-92, with evidence for two new serotypes. Epidemiol Infect. 1112(1): 187-193, 1994. PubMed: 8119358 |