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微信小章| 产品名称： | Rickettsia monacensis |
|---|---|
| 商品货号： | TS213814 |
| Classification： | Rickettsiaceae, Rickettsia |
| Deposited As： | Rickettsia monacensis |
| Agent： | Rickettsia monacensis |
| Strain： | IrR/Munich |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Genome Sequenced Strain： | Rickettsia monacensis, strain IrR/Munic, complete genome. |
| Images： |  |
| Comments： | Genome sequenced by depositor |
| Effect on Host： | CPE, refractile rounding, sloughing, and degeneration |
| Recommended Host： | Vero cells (ATCC® CCL-81™) |
| Growth Conditions： | Temperature: 34°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 65-75% confluent. Remove DMSO from bacteria vial by centrifugation (180 x g for 10 minutes at 4°C). Remove supernatant and add 2 mL of fresh medium to the pellet. Pulse vortex for 1 minute. Remove medium from host cell culture and inoculate with bacteria (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1 to 1). Adsorb by centrifugation for 30 minutes at 800 x g at 22°C. Add agent growth medium following adsorption. Incubation: 6-11 days at 34°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer. A Giemsa stain may also be used to determine end of incubation. |
| Name of Depositor： | T Kurtti, University of Minnesota |
| Chain of Custody： | ATCC |
| Source： | Tick (Ixodes ricinus), Munich, Germany. Isolated on June 12, 1998. |
| Year of Origin： | 1998 |
| References： | Simser JA, et al. Rickettsia monacensis sp. nov., a spotted fever group Rickettsia, from ticks (Ixodes Ricinus) collected in a European city park. Appl Environ Microbiol 68(9): 4559-4566, 2002. PubMed: 12200314 |
| Cross References： | Nucleotide (GenBank) : NZ_LN794217.1 Rickettsia monacensis, strain IrR/Munic, complete genome. |