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微信小章| 产品名称： | Human rhinovirus 5 |
|---|---|
| 商品货号： | TS213816 |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus B |
| Agent： | Human rhinovirus 5 |
| Strain： | Norman |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | ATCC® 1927™ was derived from ATCC® 485™ following treatment with diethyl ether to remove Mycoplasma to remove mycoplasma contamination. REFERENCE STRAIN: Collaborative Report on Rhinoviruses, Nature No. 5078, 213:761, 1967. Also for types 13-17: Panel for Picornaviruses, Melnick, J. et al., Science 141:153, 1963. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | WI-38 cells (ATCC® CCL-75™) |
| Growth Conditions： | Temperature: 33.5°C Recommendations for Infection: Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33.5°C in a humidified 5% CO2 atmosphere, rocking continuously. End adsorption by adding virus growth medium. Incubation: 2-4 days at 33.5°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | VV Hamparian, RM Conant |
| Source： | Human throat washings |
| References： | Taylor-Robinson D, Tyrrell DA. Serotypes of viruses (Rhinoviruses) isolated from common colds. Lancet 1: 452-454, 1962. PubMed: 13920011 Palmerberg AC and Gern JE. Classification and evolution of human rhinoviruses. Methods Mol Biol. 1221:1-10, 2015. doi: 10.1007/978-1-4939-1571-2_1. PubMed: 25261302 |