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微信小章| 产品名称： | Murine leukemia virus |
|---|---|
| 商品货号： | TS213823 |
| Classification： | Retroviridae, Gammaretrovirus, Mammalian virus group |
| Deposited As： | Abelson Murine leukemia virus |
| Agent： | Murine leukemia virus |
| Strain： | P160 (original isolate) (B-tropic) |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | tissue, animal Bethesda Maryland, United States |
| Product Format： | frozen |
| Storage Conditions： | -70° or colder |
| Comments： | Assays for transformation: 1) NIH 3T3 cells, Nature (Lond.) 253:729, 1975;2) IL-3 factor independence on IL-3 dependent cells, Cell 41: 685,1985;3) In vitro bone marrow (Whitlock-Witte culture), Cell 32: 90,1983. Assays for transformation: 1) NIH 3T3 cells, Nature (Lond.) 253:729, 1975;2) IL-3 factor independence on IL-3 dependent cells, Cell 41: 685,1985;3) In vitro bone marrow (Whitlock-Witte culture), Cell 32: 90,1983. |
| Effect on Host： | Yes, in vitro effects: frank refractile macroscopic foci on confluent monolayers In vitro: Frank refractile macroscopic foci on confluent monolayers.xa0 Syncytia formation (plaques) on SC (ATCC CCL-165) cells.xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 In vitro: Original isolate reported to produce lymphosarcomas in BALC/c mice. Yes, in vivo effects: induces lymphosarcomas in BALB/c mice Yes, in vitro effects: syncytium formation (plaques) on XC cells |
| Recommended Host： | Recommended Host: xa0Preferred Host: SC-1 (ATCC CRL 1404) xa0Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent). xa0Recommended Virus Growth Medium: xa0Alternate Host(s): NIH 3T3 murine hematopoietic cells, rodent fibroblasts xa0Alternate Host(s): NIH 3T3 murine hematopoietic cells, rodent fibroblasts Host Range: mice, rats |
| Growth Conditions： | Temperature: 37.0°C Duration: 7-8 days |
| Cryopreservation： | Storage temperature: -70 or lower |
| Name of Depositor： | D Baltimore |
| Special Collection： | ATCC |
| Source： | tissue, animal Bethesda Maryland, United States |
| References： | Abelson HT, Rabstein LS. Influence of prednisolone on Moloney leukemogenic virus in BALB-c mice. Cancer Res. 30: 2208-2212, 1970. PubMed: 4918190 Abelson HT, Rabstein LS. Lymphosarcoma: virus-induced thymic-independent disease in mice. Cancer Res. 30: 2213-2222, 1970. PubMed: 4318922 The virus was isolated from lymph nodes of a prednisolone-treated BALB/c mouse which had been inoculated with Moloney murine leukemia virus. From the time of isolation, the isolate appears to have contained Moloney murine leukemia virus, as well as the new Abelson virus. B-tropic B-tropic |