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| 产品名称： | Human Coxsackievirus B3 |
|---|---|
| 商品货号： | TS213846 |
| Classification： | Picornaviridae, Enterovirus, Human enterovirus B |
| Deposited As： | Coxsackievirus B3 |
| Agent： | Human Coxsackievirus B3 |
| Strain： | Nancy |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Cryopreserved in 10% FBS. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | LLC-MK2 (ATCC® CCL-7.1™) |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection:xa0Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
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| Name of Depositor： | JL Melnick |
| Source： | Stool from febrile patient with minor illness, Connecticut, 1949 |
| References： | Melnick JL, Ledinko N. Immunological reactions of the Coxsackie viruses. I. The neutralization test; technic and application. J. Exp. Med. 92: 463-482, 1950. PubMed: 14778925 |
| Cross References： | Nucleotide (GenBank) : AJ295194 Human coxsackievirus B3 genomic RNA for partial polyprotein gene, isolate VR-30, ATCC. |