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| 产品名称： | Human echovirus 18 |
|---|---|
| 商品货号： | TS213848 |
| Classification： | Picornaviridae, Enterovirus |
| Agent： | Human echovirus 18 |
| Strain： | ATCC-2011-6 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | xa0-70°C or colder |
| Effect on Host： | CPE, cell rounding and refractile cells. CPE, cell rounding and refractile cells |
| Recommended Host： | Production Host: xa0LLC-MK2 Derivative (ATCC® CCL-7.1x99) LLC-MK2 Derivative (ATCC CCL-7.1) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80 to 90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted in virus growth medium to provide an optimal MOI (e.g. MOI 0.1). Adsorb 2-2.5 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20 to 30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 5 to 6 days at 37°C in a humidified 5% CO2 atmosphere |
| Name of Depositor： | ATCC |
| Source： | Clinical patient positive for Enterovirus, Virginia, 2006 |