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微信小章| 产品名称： | Tobacco mosaic virus |
|---|---|
| 商品货号： | TS213867 |
| Classification： | Tobamovirus |
| Deposited As： | tobacco mosaic tobamovirus |
| Agent： | Tobacco mosaic virus |
| Strain： | Rabbit antiserum to Tobacco mosaic virus, alkaline phosphatase conjugated |
| Applications： | rabbit antiserum against purified tobacco mosaic virus Quality control methods: ELISA Plant research |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | rabbit antiserum against purified tobacco mosaic virus Source of antibody: rabbit serum Purified IgG was conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde. |
| Product Format： | frozen |
| Comments： | rabbit antiserum against purified tobacco mosaic virus Specificity: tobacco mosaic virus Immunogen: tobacco mosaic virus Source of antibody: rabbit serum Purification methods: affinity purification Quality control methods: ELISA The conjugate was developed by cross absorbing a rabbit polyclonal antiserum with healthy tobacco plant tissue followed by affinity purification of IgG. Purified IgG was conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde. Conjugated immunoglobulin is supplied in 1mM MgCl2, 15M NaCl and 50% glycerol and 0.05% sodium azide. Store at 4C after opening. |
| Name of Depositor： | ATCC |
| Special Collection： | ATCC |
| References： | Meshi T, et al. Molecular cloning of the complementary DNA copies of the common and cowpea strains of tobacco mosaic virus RNA. Virology 118: 64-75, 1982. Powell CA. Detection of three plant viruses by dot-immunobinding assay. Phytopathology 77: 306-309, 1987. Fraile A, Garcia-Arenal F. A classification of the tobamoviruses based on comparisons among their 126K proteins. J. Gen. Virol. 71: 2223-2228, 1990. PubMed: 2230728 Hills GJ, et al. Immunogold localization of the intracellular sites of structural and nonstructural tobacco mosaic virus proteins. Virology 161: 488-496, 1987. PubMed: 3318095 Lehto K, et al. Insertion of sequences containing the coat protein subgenomic RNA promoter and leader in front of the tobacco mosaic virus 30K ORF delays its expression and causes defective cell-to-cell movement. Virology 174: 145-157, 1990. PubMed: 2294638 Garcia AF, et al. Strains and mutants of tobacco mosaic virus are both found in virus derived from single-lesion-passaged inoculum. Virology 132: 131-137, 1984. Hegg LA, et al. Recognition of the tRNA-like structure in tobacco mosaic viral RNA by ATP/CTP:tRNA nucleotidyltransferases from Escherichia coli and Saccharomyces cerevisiae. J. Biol. Chem. 265: 17441-17445, 1990. PubMed: 2211637 Gallie DR, et al. Functional analysis of the tobacco mosaic virus tRNA-like structure in cytoplasmic gene regulation. Nucleic Acids Res. 19: 5031-5036, 1991. PubMed: 1923770 |