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微信小章| 产品名称： | Clostridioides difficile (Prevot) Lawson et al. |
|---|---|
| 商品货号： | TS213909 |
| Strain Designations： | 630 |
| Isolation： | Clinical isolate Switzerland |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Shipping Information： | Frozen |
| Preceptrol®： | no |
| Genotype： | Toxinotype: 0 |
| Type Strain： | no |
| Comments： | Preparation of Clostridioides difficile strain 630 ATCC® BAA-1382-FZ™, frozen for ease of use. Lot specific titers are available upon request.
Toxinotype 0
Ribotype 012
Presence of tcdA and tcdB genes confirmed by PCR.
Binary toxin gene cdtB not amplified by PCR
Genome sequenced strain |
| Medium： | ATCC® Medium 2107: Modified Reinforced Clostridial |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Anaerobic |
| Name of Depositor： | B Wren, H Maschler |
| References： | Wust Jurg, et al. Investigation of an Outbreak of Antibiotic-Associated Colitis by Various Typing Methods. J. Clin. Microbiol. 16: 1096-1101, 1982. Sebaihia Mohammed. The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat. Genet. 38: 779-786, 2006. Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 |