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| 产品名称： | p184 |
|---|---|
| 商品货号： | TS213917 |
| Designations： | p184 |
| Species： | Homo sapiens, human |
| Applications： | The 1.7 kb EcoRI/HindIII fragment is single copy and was used to detect the BglII RFLP. |
| Vector： | Construct size (kb): 8.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 4.5 Gene product: carboxypeptidase A1 (pancreatic)(carboxypeptidase A) CPA Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 4.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | The insert contains the following sites separated by (kb)(approx): EcoRI- 1.5- BamHI- 1.2- HindIII 1.2- BglII- 0.5- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 4.4; HindIII--6.3, 2.5; PstI--6.5, 2.5; BamHI--6.8, 2.0; BamHI/HindIII--6.0, 1.6, 1.1, 0.1; EcoRI/HindIII--4.4, 2.7, 1.7. Codominant segregation of the BglII RFLP was shown in 19 cystic fibrosis families (94 individuals) and 23 CEPH families (378 individuals). The insert is the 3 flanking region of the human carboxypeptidase A (CPA) gene. The 1.7 kb EcoRI/HindIII fragment is single copy and was used to detect the BglII RFLP. Enzyme(s) not detecting polymorphism: AluI, ApaI, AvaI, BamHI, BanII, BclII, BstEII, BstNI, DdeI, DraI, EcoRI, EcoRV, HaeII, HaeIII, HindIII, KpnI, MboII, MspI, PstI, PvuII, RsaI, SacI, SspI, StyI, and others. |
| References： | Stewart EA, et al. Human carboxypeptidase A identifies a BglII RFLP and maps to 7q31-qter. Am. J. Hum. Genet. 46: 795-800, 1990. PubMed: 1969228 |