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微信小章| 产品名称： | FL83B |
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| 商品货号： | TS213926 |
| Organism： | Mus musculus, mouse |
| Tissue： | liver |
| Cell Type： | hepatocyte |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 15 to 17 days gestation |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The cells are aneuploid. Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% |
| Derivation： | FL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal mouse. |
| Genes Expressed： | cholesterol; glycogen |
| Comments： | These cells are reported to store glycogen and actively synthesize cholesterol. They have also been reported to be able to produce serum proteins. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:6 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | EH Leiter |
| Deposited As： | mouse |
| Year of Origin： | 1969 |
| References： | Breslow JL, et al. Characterization of the mouse liver cell line FL83B. Exp. Cell Res. 78: 441-453, 1973. PubMed: 4572697 FL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal mouse. A culture submitted to the ATCC in May 1988was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM -Cycline. The cells were assayed for mycoplasma by the Hoechst stain, PCR and the standard culture test after a six-week period following treatment. All tests were negative. |