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| 产品名称： | p-rPrl |
|---|---|
| 商品货号： | TS213936 |
| Designations： | p-rPrl |
| Species： | Rattus norvegicus, rat |
| Depositors： | University of California, Davis, KH Murashige, University of California, Davis |
| Applications： | For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). |
| Vector： | Construct size (kb): 5.190000057220459 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: PstI Vector end: PstI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: PstI Vector end: PstI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: rat Gene symbol: PRL Gene name: prolactin Contains complete coding sequence?: U Chromosome: 17; Localization: 17 Tissue: anterior pituitary Type of DNA: cDNA Insert end: Modification: GC tails Insert end: Modification: GC tails Insert size (kb): 0.823 Cross references: DESCRIPTION OF INSERT COMPONENT: Genome: rat Gene symbol: PRL Gene name: prolactin Contains complete coding sequence?: U Chromosome: 17; Localization: 17 Tissue: anterior pituitary Type of DNA: cDNA Insert end: Modification: GC tails Insert end: Modification: GC tails Insert size (kb): 0.823 Cross references: Insert lengths(kb): 0.8230000138282776 Tissue: anterior pituitary Gene product: prolactin PRL |
| Insert Size (kb)： | 0.823 |
| Media： | ATCC® Medium 1083: LB medium for chi 1776 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--5.8; PvuII/PstI--3.0, 1.6, 0.9. For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). The insert includes the complete open reading frame plus 5 and 3 untranslated regions. |
| References： | Cooke NE, et al. Human and rat prolactin and preprolactin cloned genes. US Patent 4,725,549 dated Feb 16 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |