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微信小章| 产品名称： | Pseudomonas aeruginosa GFP |
|---|---|
| 商品货号： | TS213969 |
| Application： | Fluorescence detection Fluorescence imaging FACS Cell infection assays In vivo localization Pathogen-host interactions assays |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -80°C or colder |
| Preceptrol®： | no |
| Type Strain： | no |
| Antibiotic Resistance： | Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 300 µg/mL ampicillin. |
| Comments： | This clone was derived from ATCC® 15692™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling. Vector: pUCP18-MCSgfpmut3 Vector size: 5.492kb Vector type: plasmid Marker: bla (ampR) Features: GFPmut3: fluorescent maker under the control of Plac promoter Origin of replication: ori from pRO1600 Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI. Double restriction sites: HindIII, PstI, SalI, XbaI. |
| Medium： | ATCC® Medium 2854: Nutrient Agar/Broth with 300 mcg/ml Ampicillin |
| Growth Conditions： | Temperature: 37°C Atmosphere: Aerobic |
| Name of Depositor： | M Barbier |
| Chain of Custody： | Modified by ATCC (Dr. M. Barbier) <-- ATCC <-- BW Holloway <-- P Rountree |
| References： | Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster. Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227 Cox CD. Effect of pyochelin on the virulence of Pseudomonas aeruginosa. Infect. Immun. 36: 17-23, 1982. PubMed: 6804387 J. Bacteriol. 80: 362, 1960. Holloway BW. Genetic recombination in Pseudomonas aeruginosa. J. Gen. Microbiol. 13: 572-581, 1955. PubMed: 13278508 Stanier RY, et al. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43: 159-271, 1966. PubMed: 5963505 Holloway BW, Cooper GN. Lysogenic conversion in Pseudomonas aeruginosa. J. Bacteriol. 84: 1321-1324, 1962. PubMed: 13961373 Nature 181: 855, 1958. . . J. Pathol. Bacteriol. 80: 448, 1960. Bradley DE. Ultrastructure of bacteriophage and bacteriocins. Bacteriol. Rev. 31: 230-314, 1967. PubMed: 4865539 Kemp MB, Hegeman GD. Genetic control of the beta-ketoadipate pathway in Pseudomonas aeruginosa. J. Bacteriol. 96: 1488-1499, 1968. PubMed: 4973125 Britigan BE, et al. Augmentation of oxidant injury to human pulmonary epithelial cells by the Pseudomonas aeruginosa siderophore pyochelin. Infect. Immun. 65: 1071-1076, 1997. PubMed: 9038317 Hovanec TA, DeLong EF. Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria. Appl. Environ. Microbiol. 62: 2888-2896, 1996. PubMed: 8702281 |