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购买须知
| 产品名称： | pSH22.o |
|---|---|
| 商品货号： | TS213971 |
| Designations： | pSH22.o |
| Species： | Homo sapiens, human |
| Depositors： | IE Scheffler |
| Vector： | Construct size (kb): 5.5 |
| Insert： | DNA: genomic Insert lengths(kb): 2.75 Tissue: DNA from hamster/human XJM12.1.3 hybrid cell line Gene product: DNA Segment, single copy D1S*3 |
| Insert Size (kb)： | 2.75 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following sites separated by (bp)(approx): SalI- 300- SacI- 200- BamHI- 800- BamHI- 375- SphI- 675- PstI- 450- HindIII. Restriction digests of the clone give the following sizes (kb): HindIII/SalI--2.75 doublet; PstI--3.4, 2.1; EcoRI--5.5; BamHI--4.4, 0.8, 0.4; XbaI--5.5. This insert is closely linked to the centromere of human chromosome 1. This probe detects a band of 7.4 kb in EcoRI-digested genomic DNA from human cell line HT1080 and in several human-hamster hybrids that contain an intact human chromosome 1. The insert was derived from a minichromosome which includes the centromere of human chromosome 1. |
| References： | Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415 Solus J, et al. Characterization of single-copy probe from vicinity of centromere of human chromosome 1. Somatic Cell Mol. Genet. 14: 381-391, 1988. PubMed: 2840744 Immo E Scheffler, personal communication |