微信小陈
微信小章
产品简介
购买须知
| 产品名称： | M13mGP1-2 mGP1-2 |
|---|---|
| 商品货号： | TS213978 |
| Designations： | M13mGP1-2 mGP1-2 |
| GenBank Number： | J02518 |
| Species： | Escherichia coli bacteriophage T7 |
| Depositors： | Harvard Medical School, CC Richardson |
| Applications： | In suitable host, produces protein RNA polymerase |
| Vector： | Construct size (kb): 10.0 |
| Insert： | DNA: genomic
Insert lengths(kb): 2.70
Gene product: RNA polymerase gene 1
Target Gene: RNA polymerase |
| Insert Size (kb)： | 2.700 |
| Media： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--10.0; HindIII--10.0; EcoRI/HindIII--7.2, 2.8; XbaI--uncut. This construct contains nt 3133-5840 (gene 1) of bacteriophage T7. M13mGP1-2 (TS213978) can coexist in the same cell as the two plasmids of ATCC 67287, pGP5-5 and pTrx-2. The T7 RNA polymerase of M13mGP1-2 can be used to regulate expression of T7 DNA polymerase by inducing the RNA polymerase with IPTG. M13mGP1-2 (TS213978) is similar to pGP1-2 (ATCC 40175) except that gene 1, T7 RNA polymerase, was ligated (using BglII and SalI linkers) to BamHI-SalI cut M13mp8, placing the gene under control of the lac promoter. |
| References： | Tabor S, Richardson CC. T7 DNA polymerase. US Patent 4,795,699 dated Jan 3 1989 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |