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| 产品名称： | p13-1-25 |
|---|---|
| 商品货号： | TS213981 |
| Designations： | p13-1-25 |
| Species： | Homo sapiens, human |
| Applications： | Can use the entire plasmid as probe with standard hybridization and washing conditions. |
| Vector： | Construct size (kb): 7.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 2.0 Gene product: DNA Segment, single copy D19S11 Alleles: F1, F2, A1, A1, A2, A2, B1, B2, C1, C2, D1, D2, D3 |
| Insert Size (kb)： | 2.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--5.7, 1.2, 0.56, 0.28; Sau3A--1.7, 0.88, smaller fragments; HindIII: 7.4; PstI--5.0, 2.4; SalI--7.4. EcoRI cuts the insert twice giving fragments of 1.2, 0.54 and 0.28 kb. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. This probe detects 3 different TaqI polymorphisms. Alpha (C alleles) is the same detected by p13-1-82 (ATCC 59004, 59005). Epsilon has more than 32 alleles. Phi (F alleles) has 2 common alleles. The combined heterozygosity for these is 98%. The highly polymorphic epsilon polymorphism includes a relatively frequent null allele. Can use the entire plasmid as probe with standard hybridization and washing conditions. Use a final wash of 0.1X SSC/0.1% SDS at 65C. p13-1-82 (ATCC 59004, 59005), p13-2-31 (ATCC 59014, 59015), and p13-1-25 (ATCC 59870, 59871) were subcloned from cosmid 1-13 (C1-13). |
| References： | Buroker NE, et al. A hypervariable region at the D9S11 locus. Hum. Genet. 76: 90-95, 1987. PubMed: 2883111 Michael Litt, personal communication |