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微信小章| 产品名称： | RK13 |
|---|---|
| 商品货号： | TS213990 |
| Organism： | Oryctolagus cuniculus, rabbit |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 5 weeks |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | keratin |
| Cellular Products： | keratin |
| Virus Susceptibility： | Herpes simplex virus
Pseudorabies virus Vaccinia virus Rabbitpox virus Myxoma virus Simian adenovirus 11 Simian adenovirus 17 Simian adenovirus 3 Simian adenovirus 1 Simian adenovirus 20 Simian adenovirus 18 Simian adenovirus 16 Simian adenovirus 8 Simian adenovirus 19 Simian adenovirus 21 Simian adenovirus 25 Simian adenovirus 22 Simian adenovirus 23 Simian adenovirus 38 Simian adenovirus 37 Simian adenovirus 27 Simian adenovirus 27 Simian adenovirus 39 Simian adenovirus 32 Simian adenovirus 34 Simian adenovirus 31 Simian adenovirus 33 Simian adenovirus 36 Rubella virus , Rubella virus |
| Comments： | The cells are positive for keratin by immunoperoxidase staining. The cells are positive for bovine viral diarrhea virus (BVDV). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | BC Meyer |
| Deposited As： | Oryctolagus cuniculus |
| References： | Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 Beale AJ, et al. Rabbit Cells Susceptible to Rubella Virus. The Lancet 282: 640-641 1963 Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 McCarthy K, et al. Isolation of Rubella Virus from Cases in Britain. The Lancet 282: 593-598 1963. PubMed: 14050876 Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 |