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| 产品名称： | pSCH2012 |
|---|---|
| 商品货号： | TS213994 |
| Designations： | pSCH2012 |
| GenBank Number： | M98270 |
| Species： | Pseudomonas aeruginosa (Schroeter) Migula |
| Depositors： | KJ Shaw |
| Vector： | Construct size (kb): 3.150000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.2039999961853027 Gene product: aminoglycoside-4-adenyltransferase ant(4)-IIa |
| Insert Size (kb)： | 0.204 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.0; PstI--3.0; HindIII/PstI--2.9, 0.2. The insert contains the following restriction sites (approximate kb from the 5 end): ClaI--0.01; PvuII--0.18 A single gel purification of the PCR generated probe is necessary since flanking regions will co-amplify with the gene specific sequence. Failure to do so often results in high backgrounds and false positives with clinical E. coli strains. A hybridization probe may be generated using the following vector specific PCR primers: modified T3 = 5-CCCCTCACTAAAGGGAACAAAAGCTG-3 and modified T7 = 5-CGCGTAATACGACTCACTATAGGGCGAA-3. |
| References： | Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996 |