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微信小章| 产品名称： | EM9 (DNA repair mutant of CHO) |
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| 商品货号： | TS213996 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial-like |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | EM9 is a repair deficient mutant derived from AA8 (see ATCC CRL-1859). The line is defective in single strand break repair, has a 10 fold higher baseline frequency of sister chromatid exchange relative to AA8 and a 2 fold greater sensitivity to killing by X-rays. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61). EM9 is a repair deficient mutant derived from AA8 (see ATCC CRL-1859). |
| Clinical Data： | female |
| Comments： | This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61). EM9 is a repair deficient mutant derived from AA8 (see ATCC CRL-1859). The line was selected for enhanced sensitivity to ethylmethanesulfonate (EMS). The line is defective in single strand break repair, has a 10 fold higher baseline frequency of sister chromatid exchange relative to AA8 and a 2 fold greater sensitivity to killing by X-rays. This defect is corrected by the human XRCC1 gene. |
| Complete Growth Medium： | Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:12 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | LH Thompson |
| References： | Thompson LH, et al. A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange. Mutat. Res. 95: 427-440, 1982. PubMed: 6889677 Thompson LH, et al. A screening method for isolating DNA repair-deficient mutants of CHO cells. Somatic Cell Genet. 6: 391-405, 1980. PubMed: 7404270 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |