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微信小章| 产品名称： | RBL-1 |
|---|---|
| 商品货号： | TS214039 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | peripheral blood |
| Cell Type： | chemically-induced basophil |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | leukemia |
| Strain： | Wistar |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Receptor Expression： | FcERI (Fc of IgE) |
| Comments： | This cellxa0line exhibits various characteristics of basophil differentiation including surface receptors for IgE. It was shown to not release histamine by an IgE mediated system. Ref   Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | xa0Cultures can be maintained by the addition or replacement of fresh medium. Start cultures at 2xa0x 105 viable cells/mL and maintain between 1xa0x 105 cells/mL and 1xa0x 106 /mL.
Medium Renewal: Add medium as cell density increases |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | H Metzger, C Isersky |
| Deposited As： | Rattus sp. |
| References： | Kulczycki A Jr., et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. PubMed: 4812630 Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481 Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973. |
| Cross References： | Nucleotide (GenBank) : NM_031580 Rattus norvegicus glucose regulated protein, 58 kDa (Grp58), mRNA. Nucleotide (GenBank) : X12355 Rat mRNA for phosphoinositide-specific phospholipase C form-I (PI-PLC I). |