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| 产品名称： | pGEM-8 |
|---|---|
| 商品货号： | TS214066 |
| Designations： | pGEM-8 |
| GenBank Number： | J03528 |
| Species： | Homo sapiens, human |
| Depositors： | WS Sly |
| Vector： | Construct size (kb): 12.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-2 Intact vector size: 2.870 Type of vector: plasmid Vector end: SalI Vector end: XbaI Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): MCS: EcoRI...HindIII, ->, 10-60 promoter for in vitro transcription: T7, replicon: pMB1 marker(s): ampR, promoter for in vitro transcription: SP6, ->, 2852-2869 Cross references: DNA Seq. Acc.: X65301 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IGF2R Genomic copy number: unique Gene name: insulin-like growth factor 2 receptor Contains complete coding sequence?: Y Chromosome: 6; Localization: 6 q25.3 Tissue: placenta Type of DNA: cDNA Insert 5 end: SalI Insert 3 end: XbaI Insert size (kb): 9.1 Cross references: DNA Seq. Acc.: J03528 Insert lengths(kb): 9.100000381469727 Tissue: placenta Gene product: insulin-like growth factor 2 receptor( insulin-like growth factor II receptor, insulin like growth factor II (IGF II) receptor, mannose-6-phosphate receptor, cation-independent) IGF2R Alleles: A1, A1 |
| Insert Size (kb)： | 9.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.0, 1.8; EcoRI--8.0, 3.7; HindIII--5.0, 4.0, 1.8, 1.5; SalI--9.0, 2.7; SalI/XhoI--9.0, 2.7. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.48, 8.40; HindIII--0.72, 2.24, 3.94; BamHI--2.81, 2.96, 3.34, 6.91. At the 3 end of the insert, the construct includes sequence from the vector pSLV: XbaI, SmaI, SacI, and BamHI sites, and the SV40 poly(A) polyadenylation signal (to the SalI site). |
| References： | Oshima A, et al. The human cation-independent mannose 6-phosphate receptor. Cloning and sequence of the full-length cDNA and expression of functional receptor in COS cells. J. Biol. Chem. 263: 2553-2562, 1988. PubMed: 2963003 William S Sly, personal communication |