微信小陈
微信小章| 产品名称： | HK-PEG-1 formerly PEG 1-6 |
|---|---|
| 商品货号： | TS214071 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Influenza |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | The line was produced by fusing P3X63Ag8 myeloma cells with spleen cells from BALB/c mice that had been immunized with influenza virus (A.PR/8/34). |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | The hybridoma cell line HK-PEG-1 secretes a mouse monoclonal antibody (IgG3) that reacts with influenza virus.xa0 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium.
Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 to 8 x 104xa0viable cells/mL. Maintain cell density between 1 x 105xa0and 1 x 106xa0viable cells/mL.
Medium Renewal: Add fresh medium (depending on cell density) every 2-3 days. |
| Cryopreservation： | Complete growth medium supplemented with 10% (v/v) DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature:xa037°C |
| Isotype： | mouse IgG3 |
| Name of Depositor： | WU Gerhard |
| U.S. Patent Number： | |
| References： | Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |