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微信小章| 产品名称： | BJ |
|---|---|
| 商品货号： | TS214085 |
| Organism： | Homo sapiens, human |
| Tissue： | skin; foreskin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | newborn |
| Gender： | male |
| Applications： | The cells may be used for stable transfection studies. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The line was established from skin taken from normal foreskin. |
| Clinical Data： | newborn
male |
| Comments： | The BJ cell line has a long lifespan in comparison with other normal human fibroblast cell lines.
A frozen ampule at population doubling 2.3 was received at the ATCC in April, 2000.
These cells have a reported normal diploid karyotype at population doubling 61 but an abnormal karyotype at population doubling 82.
They are telomerase negative.
Cells from ATCC ampules have the capacity to proliferate to a maximum of 72 population doublings before the onset of senescence. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:9 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,9 D16S539: 9,13 D5S818: 12 D7S820: 11,12 THO1: 7,8 TPOX: 10,11 vWA: 16,18 |
| Population Doubling Capacity： | Cells from ATCC ampules have the capacity to proliferate to a maximum of 72 population doublings before the onset of senescence. |
| Name of Depositor： | JR Smith |
| Deposited As： | human |
| Passage History： | A frozen ampule at population doubling 2.3 was received at the ATCC in April, 2000. |
| References： | Yi X, et al. Both transcriptional and posttranscriptional mechanisms regulate human telomerase template RNA levels. Mol. Cell. Biol. 19(6): 3989-3997, 1999. PubMed: 10330139 Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 Huffman KE, et al. Telomere shortening is proportional to the size of the G-rich telomeric 3-overhang. J. Biol. Chem. 275: 19719-19722, 2000. PubMed: 10787419 Morales CP, et al. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase. Nat. Genet. 21: 115-118, 1999. PubMed: 9916803 |