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微信小章| 产品名称： | B11F7 |
|---|---|
| 商品货号： | TS214089 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | B lymphocyte; hybridom |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts well with denatured MAG, and is excellent for western blots and immunostaining of fixed tissue. The B11F7 monoclonal antibody recognizes a polypeptide epitope and is highly specific for MAG of most species. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Animals were immunized with human myelin-associated glycoprotein (MAG) isolated from central nervous system myelin. Spleen cells from immunized mice were fused with P3X63Ag8.653 mouse myeloma cells and the resulting MAG antibody secreting hybrids were identified using the ELISA assay. The B11F7 monoclonal antibody recognizes a polypeptide epitope and is highly specific for MAG of most species. Immunocytochemical staining of adult human spinal cord with the antibody resulted in periaxonal staining of myelin sheaths. The antibody reacts well with denatured MAG, and is excellent for western blots and immunostaining of fixed tissue. However, it does not bind to native MAG, so it does not immunostain live cells or block function. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against protein backbone of human and rat myelin-associated glycoprotein (MAG) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against protein backbone of human and rat myelin-associated glycoprotein (MAG) |
| Comments： | Animals were immunized with human myelin-associated glycoprotein (MAG) isolated from central nervous system myelin. Spleen cells from immunized mice were fused with P3X63Ag8.653 mouse myeloma cells and the resulting MAG antibody secreting hybrids were identified using the ELISA assay. The B11F7 monoclonal antibody recognizes a polypeptide epitope and is highly specific for MAG of most species. Immunocytochemical staining of adult human spinal cord with the antibody resulted in periaxonal staining of myelin sheaths. The antibody reacts well with denatured MAG, and is excellent for western blots and immunostaining of fixed tissue. However, it does not bind to native MAG, so it does not immunostain live cells or block function. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 to 1 x 105 viable cells/ mL. Maintain cultures at a cell concentration between 5 x 105 and 1 x 106 cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2b, kappa |
| Name of Depositor： | RH Quarles |
| Year of Origin： | 1984 |
| References： | Doberson MJ, et al. Generation and characterization of mouse monoclonal antibodies to the myelin-associated glycoprotein (MAG). Neurochem. Res.;10(4):499-513, 1985. PubMed: 2582290 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |