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| 产品名称： | puc19TM15 lambda HTm15 |
|---|---|
| 商品货号： | TS214107 |
| Designations： | puc19TM15 lambda HTm15 |
| GenBank Number： | M16552 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.700000047683716 Tissue: umbilical vein endothelial cells Gene product: thrombomodulin THBD |
| Insert Size (kb)： | 3.700 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert contains the following sites separated by (bp)(approx): EcoRI- 270- SmaI- 160- PstI- 240- PstI- 210- PstI- 100- PstI- 530- KpnI- 310- PstI- 1080- HindIII- 110- BamHI- 205- PstI- 475- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.7; SacI--6.5; AvaI--3.4, 2.0, 0.2; XbaI--5.9, 0.54, 0.2, 0.1. The insert includes 146 nt of 5-noncoding sequence, an open reading frame of 1725 nt and 1779 nt of 3-noncoding sequence including 40 nt of poly(A) tail. There is 64% homology between this probe and bovine thrombomodulin. A single band of 3.7 kb is detected in human placenta and endothelial cell poly(A)+ RNA. |
| References： | Wen DZ, et al. Human thrombomodulin: complete cDNA sequence and chromosome localization of the gene. Biochemistry 26: 4350-4357, 1987. PubMed: 2822087 Espinosa R, et al. Regional localization of the human thrombomodulin gene to 20p12-cen. Genomics 5: 649-650, 1989. PubMed: 2559025 Scarpati EM, Sadler JE. Regulation of endothelial cell coagulant properties. J. Biol. Chem. 264: 20705-20713, 1989. PubMed: 2555368 |