微信小陈
微信小章| 产品名称： | Porcine reproductive and respiratory syndrome virus |
|---|---|
| 商品货号： | TS214142 |
| Classification： | Arteriviridae, Arterivirus |
| Deposited As： | Porcine respiratory and reproductive syndrome virus |
| Agent： | Porcine reproductive and respiratory syndrome virus |
| Strain： | BIAH-001 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Genome Sequenced Strain： | Porcine reproductive and respiratory syndrome virus strain TS214142, complete genome |
| Images： |  |
| Comments： |
Virus may cause disease in swine, specifically respiratory disease and stillbirth in pregnant sows.xa0 The virus is a fastidious, non-hemagglutinating enveloped RNA virus.xa0 It is heat labile and grows best at 34°C to 35°C.xa0 Media should be equilibrated in a 5% CO2 incubator overnight before use.xa0 Once fed, virus must be kept in sealed flask or container that prevents further exchange of CO2 with culture.xa0 Inoculate 80-90% confluent monolayer of cell. Note: This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims. |
| Effect on Host： | CPE, cell rounding and sloughing. CPE progresses rapidly once it starts. |
| Recommended Host： |
MA-104 Clone 1 cells (ATCC® CRL-2378.1™)
|
| Growth Conditions： | Temperature: 35°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation:xa02-5 days at 35°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | Boehringer Ingelheim Corp. |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | Combined tissues obtained from a SIRS infected piglet |
| References： | Chaladek DW, et al. Method of growing and attenuating a viral agent associated with mystery swine disease. US Patent 5,476,778 dated Dec 19 1995 |
| Cross References： | Nucleotide (GenBank) : U87392.3 Porcine reproductive and respiratory syndrome virus strain TS214142, complete genome |