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微信小章| 产品名称： | NFS-70 C-10 |
|---|---|
| 商品货号： | TS214192 |
| Organism： | Mus musculus, mouse |
| Cell Type： | pro-B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | NFS/N |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The NFS-70 C-10 cell line was derived from a lymphoma arising in a mouse inoculated with CAS-NS-7 ecotropic murine leukemia virus. |
| Antigen Expression： | CD5 +; CD32 +; CD45R + |
| Genes Expressed： | CD5 +; CD32 +; CD45R + |
| Comments： | The NFS-70 C-10 cell line was derived from a lymphoma arising in a mouse inoculated with CAS-NS-7 ecotropic murine leukemia virus. The cells bear markers of pro-B lymphoblasts (Mac 1, CD11b. Ly 17, CD32. Ly 1, CD5. Lyb 2, Ly 5(B220), CD45R). They are negative for ThB, surface immunoglobulin (sIg) and Ia. The cells appear to be at a very early stage of commitment to B cell differentiation. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Modified Dulbeccos medium with 0.002 mg/ml transferrin and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly
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| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | WJ Davidson, HC Morse |
| Deposited As： | Mus musculus |
| References： | Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |