微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pJ6omega |
|---|---|
| 商品货号： | TS214209 |
| Designations： | pJ6omega |
| Depositors： | H Land |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.5000000000000000 Vector: pJ6omega (plasmid) Promoters: Promoter rat beta-actin Construction: pBR322, SV40, rat beta-actin Marker(s):ampR Construct size (kb): 3.5 Features: marker(s): ampR promoter: rat beta-actin replicon: pMB1 terminator: SV40 |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; BamHI--3.5; SmaI--3.5; PvuI--2.15, 1.35. The actin promoter has been shown to be expressed efficiently in undifferentiated cells in which transcription from other promoters (SV40 early, Mo MuLV LTR, MMTV LTR) is severely repressed. A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the rat beta actin promoter. Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and the rat beta-actin promoter (-340 to +10). |
| Media： | ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225 Tsutsumi M, et al. Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor. Mol. Endocrinol. 6: 1163-1169, 1992. PubMed: 1324422 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |