微信小陈
微信小章| 产品名称： | MDA-MB-415 |
|---|---|
| 商品货号： | TS214221 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland/breast; derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 38 years |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number =73; range = 65 to 76. The stemline chromosome number is hypertriploid with the 2S component occurring at 0.8%. Ten markers (del(2), t(6;?), t(6;?), t(2;12), t(14;?), and single or paired M6, M7, M8, M9, and M10) were common to all cells, and four others (M13, M15, M16, and t(13,21) occurred in some cells. The X chromosome was generally tetrasomic and chromosome 4 was tetrasomic or pentasomic (P35). No Y chromosomes were detected in QM stained preparations. |
| Images： |  |
| Clinical Data： | female
Caucasian |
| Antigen Expression： | Blood Type O |
| Genes Expressed： | amelogenin (X chromosome)(AMELEX) |
| Tumorigenic： | No |
| Effects： | Yes, in semisolid medium No, in immunosuppressed mice |
| Comments： | The cells express the WNT7B oncogene PubMed: 8168088. The patient presenting with the tumor was from Paraguay and, although listed as Caucasian, may have had mixed ancestry as suggested by the presence of G6PD type A phenotype in the cells. The line forms flat, spreading plaques of epithelia which exhibit desmosomes, extensive microtubules and microfilaments upon examination with the electron microscope.xa0 It is not amenable to trypsinization. |
| Complete Growth Medium： | Leibovitzs L-15 medium with 2mM L-glutamine supplemented with 10 mcg/ml insulin and 10 mcg/ml glutathione, 85%; fetal bovine serum, 15%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 11,13 D16S539: 13 D5S818: 11,13 D7S820: 9,10 THO1: 7,9.3 TPOX: 8 vWA: 17 |
| Isoenzymes： | AK-1, 1 ES-D, 2 G6PD, A GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | R Cailleau |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1975 |
| References： | Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 Beall PT, et al. Polypeptides that inhibit human breast cancer cell division. Cancer Biochem. Biophys. 3: 93-96, 1979. PubMed: 552902 Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 |