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| 产品名称： | pEBA-6 |
|---|---|
| 商品货号： | TS214224 |
| Designations： | pEBA-6 |
| Depositors： | EW Miles |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.5399999618530270 Vector: pEBA-6 (plasmid) Promoters: Promoter Ptrc Construction: pCR2 Marker(s):TRP1,ampR Construct size (kb): 5.539999961853027 Features: primer site: 5-TTGACAATTAATCATCCGGC-3 promoter: Ptrc terminator: rrnBT1 terminator: rrnBT2 |
| Applications： | contains sequence |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.5; EcoRI--5.5; PstI--5.5; EcoRI/PstI--4.2, 1.4. Overproduces the beta2 subunit of tryptophan synthase from Salmonella typhimurium. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yang LH, et al. PCR mutagenesis and overexpression of tryptophan synthase from Salmonella typhimurium: on the roles of beta2 subunit Lys-382. Protein Expr. Purif. 8: 126-136, 1996. PubMed: 8812843 Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046 |
| Related Products： | component of:ATCC 87809 |
| Shipped： | frozen |