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| 产品名称： | Bluegill virus |
|---|---|
| 商品货号： | TS214236 |
| Classification： | Reoviridae, Reovirus |
| Agent： | Bluegill virus |
| Strain： | EFDL Clone B |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Dermal scrapings from bluegill (fish) (Lepomis macrochirus) |
| Product Format： | frozen |
| Storage Conditions： | -80°C |
| Comments： | The only cells thus far known to replicate this agent are those from fishes, but there is an additional restriction: cells with a fibroblast-like morphology are required. The agent is not replicated by fathead minnow cells (ATCC CCL 42). Previously believed to be a paramyxovirus. |
| Effect on Host： | elongate pyknosis followed by lysis |
| Recommended Host： | bluegill fry (BF-2) cells (ATCC CCL-91) BF-2 cells (ATCC CCL-91); RTG-2 cells (ATCC CCL-55) |
| Growth Conditions： | Temperature: 25°C Incubation: 3 days |
| Name of Depositor： | KE Wolf, MC Quimby |
| Special Collection： | ATCC |
| Source： | Dermal scrapings from bluegill (fish) (Lepomis macrochirus) |
| References： | Hoffman GL, et al. Epitheliocystis, a new infectious disease of the bluegill (Lepomis macrochirus). Antonie van Leeuwenhoek 35: 146-158, 1969. PubMed: 4987372 Wolf K, Quimby MC. Fish viruses: buffers and methods for plaquing eight agents under normal atmosphere. Appl. Microbiol. 25: 659-664, 1973. PubMed: 4349252 |