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| 产品名称： | pSL7 |
|---|---|
| 商品货号： | TS214242 |
| Designations： | pSL7 |
| Depositors： | T Lufkin |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.4000000953674320 Vector: pSL7 (plasmid) Construction: pBS Marker(s):ampR Construct size (kb): 6.400000095367432 Features: marker(s): ampR other: internal ribosome entry sequence (IRES) replicon: pMB1 reporter group: alkaline phosphatase terminator: SV40 polyadenylation |
| Applications： | reporter construct alkaline phosphatase, placental (Regan isozyme) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 2.5, 0.6, 0.2; HindIII--5.5, 0.6, 0.3; SpeI--6.4. Plasmid expressing alkaline phosphatase. Foreign genes can be cloned upstream of the IRES/reporter gene, allowing production of a dicistronic messenger RNA in mammalian cells. IRES/reporter insert lacking polyA can be excised with NruI. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Li X, et al. Dicistronic lacZ and alkaline phosphatase reporter constructs permit simultaneous histological analysis of expression from multiple transgenes. BioTechniques 23: 874-882, 1997. PubMed: 9383553 Thomas Lufkin, personal communication |
| Shipped： | freeze-dried |