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| 产品名称： | pSCH2003 plasmid in E. coli |
|---|---|
| 商品货号： | TS214246 |
| Designations： | pSCH2003 plasmid in E. coli |
| GenBank Number： | X04555 |
| Species： | Unidentified bacterium |
| Depositors： | KJ Shaw |
| Vector： | Construct size (kb): 3.25 |
| Insert： | DNA: Synthetic Insert lengths(kb): 0.305 Gene product: aminoglycoside adenylyltransferase(spectinomycin resistance, streptomycin resistance) ant(2")-Ia |
| Insert Size (kb)： | 0.305 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--3.3; HindIII--3.3; PstI--3.3; HindIII/XhoI--2.8, 0.4. The insert contains the following restriction sites (approximate kb from the 5 end): PvuII--0.20; RsaI--0.25; SphI--0.29. A single gel purification of the PCR generated probe is necessary since flanking regions will co-amplify with the gene specific sequence. Failure to do so often results in high backgrounds and false positives with clinical E. coli strains. A hybridization probe may be generated using the following vector specific PCR primers: modified T3 = 5-CCCCTCACTAAAGGGAACAAAAGCTG-3 and modified T7 = 5-CGCGTAATACGACTCACTATAGGGCGAA-3. |
| References： | Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996 |