微信小陈
微信小章| 产品名称： | HCC1395 BL |
|---|---|
| 商品货号： | TS214303 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast; Epstein-Barr virus (EBV) transformed |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 2 Cells contain Epstein-Barr Virus (EBV)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 43 years adult |
| Gender： | female |
| Ethnicity： | Caucasian, White |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | HCC1395 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as the breast carcinoma cell line HCC1395.xa0 The cell line was initiated from a 43-year-old white female with a TNM stage I, grade 3 primary ductal carcinoma and a prior history of cancer; her mother had breast cancer. |
| Clinical Data： | 43 years
female
Caucasian |
| Comments： | This cell line is also available at ATCC as (ATCC CRL-2322). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | AF Gazdar, AK Virmani |
| References： | Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |