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| 产品名称： | pPD101 |
|---|---|
| 商品货号： | TS214312 |
| Designations： | pPD101 |
| Depositors： | E Bremer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.5199999809265140 Vector: pPD101 (plasmid) Promoters: Promoter T7 gene 10 Construction: pHSG576, pT7-6, pUC-BM20 Marker(s):cmlR Construct size (kb): 3.519999980926514 Features: marker(s): cmlR promoter: T7 gene 10 replicon: pSC101 MCS: HindIII...EcoRI |
| Applications： | expression vector mobilization helper plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; NcoI--2.7, 0.7. pPD100 (ATCC 87222) and pPD101 (TS214312) require that the insert provide a translational initiation signal for expression. pPD100 differs from pPD101 only in the orientation of the MCS. Deposited for research purposes only. Before any commercial use, the depositor must be contacted. A low copy number plasmid using the T7 gene 10 promoter for selective, overexpression of cloned genes in E. coli. |
| Media： | ATCC® Medium 2272: LB Medium (Miller) with chloramphenicol 25 mcg/ml |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Dersch P, et al. Low-copy-number T7 vectors for selective gene expression and efficient protein overproduction in Escherichia coli. FEMS Microbiol. Lett. 123: 19-26, 1994. PubMed: 7988888 Erhard Bremer, personal communication |
| Shipped： | freeze-dried |