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微信小章| 产品名称： | CRE BAG 2 |
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| 商品货号： | TS214317 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Leukemia |
| Strain： | NIH/Swiss |
| Applications： | produces protein beta-galactosidase beta-D-galactosidase |
| Derivation： | The CRE BAG 2 cell line has never produced a replication competent helper virus. The cells will package vectors derived from murine leukemia viruses. |
| Genes Expressed： | a beta galactosidase transducing vector (BAG),beta-galactosidase(beta-D-galactosidase) lacZ,beta-galactosidase |
| Cellular Products： | a beta galactosidase transducing vector (BAG) |
| Comments： | The BAG construct has been used to transfect psiCRE cells in TS214317 and to transfect psi2 cells in ATCC CRL-9560. The CRE BAG 2 cell line has never produced a replication competent helper virus. This line produces a vector (BAG) that can infect mouse and rat cells and transduce beta-galactosidase activity. The line is similar to the psi 2 BAG alpha line (see ATCC CRL-9560) but has never been known to produce a replication competent helper virus. The cells will package vectors derived from murine leukemia viruses. The vector has been used to infect a variety of neural cell types and to express beta-galactosidase and resistance to G418. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
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| Subculturing： | Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin - 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | CL Cepko |
| References： | Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292 Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789 Danos O, Mulligan RC. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85: 6460-6464, 1988. PubMed: 3413107 Cepko CLVirus propagation and Xgal proceduresIn: Cepko CLNeuromethodsClifton, NJHumana Press1989. Le Doux JM, et al. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J. Virol. 70: 6468-6473, 1996. PubMed: 8709284 |