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微信小章| 产品名称： | pMT2-VWF |
|---|---|
| 商品货号： | TS214322 |
| Designations： | pMT2-VWF |
| GenBank Number： | X04385 |
| Species： | Homo sapiens, human |
| Depositors： | Genetics Institute, Inc. |
| Applications： | in another host, produces protein von Willebrand factor coagulation factor VIII VWF (von Willebrand factor) |
| Vector： | Construct size (kb): 12.77499961853027 |
| Insert： | DNA: cDNA Insert lengths(kb): 8.574999809265137 Gene product: von Willebrand factor(coagulation factor VIII VWF (von Willebrand factor)) VWF Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2 Target Gene: von Willebrand factor |
| Insert Size (kb)： | 8.575 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Frozen E. coli containing the plasmid |
| Comments： | The insert contains the following restriction sites (nt position according to the published sequence): SacII--1614, 1991, 7618; BglII--2240, 6935; HindIII--2334; BamHI--2816. Restriction digests of the clone give the following sizes (kb): EcoRI--8.6, 5.5; BamHI--6.7, 3.7, 3.25, 0.5; BglII--7.0, 4.9, 2.35; HindIII--10.8, 2.5, 0.95. Contains the complete coding sequence (nt 100 to nt 8541), with the mature peptide encoded from nt 2389 to nt 8538. May be transfected into COS cells for transient expression of an immunoreactive product detected in conditioned medium. The vector contains the SV40 ori, the adenovirus major late promoter and tripartite leader, the SV40 polyadenylation signal, the adenovirus VA I and VA II genes, and pBR322 sequences. |
| References： | Bonthron DT, et al. Structure of pre-pro-von Willebrand factor and its expression in heterologous cells. Nature 324: 270-273, 1986. PubMed: 3491324 Wise RJ, et al. The propeptide of von Willebrand factor independently mediates the assembly of von Willebrand multimers. Cell 52: 229-236, 1988. PubMed: 3124962 Bonthron D, et al. Nucleotide sequence of pre-pro-von Willebrand factor cDNA. Nucleic Acids Res. 14: 7125-7127, 1986. PubMed: 3489923 DAndrea A, et al. Recombinant DNA encoding an erythropoietin receptor. US Patent 5,278,065 dated Jan 11 1994 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |